Journal of Cancer Therapeutics and Immunotherapy

ABSTRACT

Present study investigates anti-proliferative and cytotoxic e­ffect of chloroform extract of Shorea robusta plant resin on murine and human cancer cell lines. The plant resin powder sample was collected after air dried and extracted by cold percolation method in chloroform and column fractionate the chemical constituents into individual fractions. The confirmation of compounds present with di­erent solvents by using preparative and analytical Thin Layer Chromatography (TLC) methods. Cytotoxic eff­ects of chloroform extract of S. robusta plant resin were analyzed through various of morphological changes like Trypan Blue exclusion assay, 3-(4,5-dimethylthiazol-2-yl)- 2,5-diphenyltetrazolium bromide assay (MTT) and4’,6-di-amidino-2-phenylindole (DAPI) Test. Apoptotic or necrotic cell death by Acridine Orange/Ethidium Bromide (AO/EtBr) double staining was investigated. We found that the half-maximal inhibitory concentration (IC50) value of S. robusta resin was 163.94, 120.57, 144.89, 189.54. Hence extract of (70%) chloroform of S. robusta resin sample was used for its anti-inflammatory and anti-proliferative actions, which induced apoptosis in cancer cell lines.

KEYWORDS

1. Shorea robusta
2. Anti-proliferative
3. Cytotoxicity
4. Chloroform
5. Resin
6. Cancer