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The present investigation is focused on bryophytes association for the characterization of silver nanoparticles, LC-MS and enzyme activity of the liverwort Targionia hypophylla L. and two mosses as Trachypodopsis blanda (Mitt.) Fleisch. and Stereophyllum anceps (Bosch and Lac.) Broth from the Khambataki ghat Khandala and Wai, Satara of Maharashtra, India. Nanotechnology is useful to solving different environmental and health problems of human beings which is also useful for chemical fertilizers in agriculture aspects. Nanoparticles are useful in increasing the growth and production of crops. Synthesis of nanoparticles change in colour was observed from pale yellow to finally reddish brown. The peak is between 400nm to 440nm was acquired on a UV Visible Spectrophotometer which established silver nanoparticles. LC-MS is an analytical chemistry technique that combines liquid chromatography and mass spectroscopy which is useful for the analysis of metabolites like Phenol. In S. anceps phenol is present named carvacrol. In Targionia hypophylla is a tricyclic glycopeptide antibiotic named Vancomycin. Study of enzymes like Amylase and Nitrate reductase. Amylase is a group of enzymes that reduce starch into simple sugar. There are valuable industrial enzymes, a major resource of biologically active compounds in bryophytes. Another enzyme is nitrate reductase is the first enzyme of the nitrogen reduction pathway in plants. The production of ammonia plays a crucial role in nitrogen-fixing symbiosis, particularly in bryophytes.