Original Article

Impact of a combination of NPK and urea fertilizers on bioremediation of soil contaminated with crude oil

Abstract

This research, centered on the impact of a combination of inorganic fertilizers (NPK and Urea) on bioremediation as an alternative of the use of solely one fertilizer. The effect of bio-stimulation on crude oil degradation in contaminated soil was investigated in six therapy group and C:N:P ratio of 100:2:0.2 were used. The microbial growths were measured as total heterotrophic bacteria, total hydrocarbon utilizing bacteria, total heterotrophic fungi and total hydrocarbon utilizing fungi. Laccase, Peroxidase, Lipase and Catalase activities as well as residual petroleum hydrocarbon were assayed every 6 days for 36 day. From this study, the outcomes acquired from the chemical analyses published that water holding capacity, moisture content, bulk density were higher in the treated soil; while organic carbon, electrical conductivity were higher in contaminated soil and porosity, whole nitrogen and complete accessible phosphorus are higher in uncontaminated soil. The study also shows that treatment with the inorganic fertilizer increased the activities of soil enzymes, soil microbial load and decreased in total petroleum hydrocarbon. The effects acquired from this findings suggests that combination of inorganic fertilizers and C:N:P ratio adopted shortened the time frame for bioremediation of crude oil in opposition to when only NPK is been applied at 10% as suggested via other researchers.

Keywords

NPK fertilizerUrea fertilizerBioremediationSoil microbe’sm Soil enzymesCrude oil contamination

Corresponding Author

Dr. May Chidinma Ilonzeh

Department of Biochemistry, University of Benin, Edo State, Nigeria

mayilonzeh@gmail.com

Article History

Received Date : 12 October 2024

Revised Date : 07 November 2024

Accepted Date : 27 December 2024

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